4/3/2023 0 Comments Capto adhere chromatography![]() ![]() For optimal separation use approximately one fifth of the total binding capacity.Protein binding capacity is protein-to-protein dependent. The binding capacity values in the media and column instructions, there are examples and to be considered as starting values.Gel filtration columns and media selection guide and product profile selection guide. Sample preparation for analysis of proteins, peptides and carbohydrates selection guide. Regarding sample volumes for prepacked columns, please refer to selection guide below:.Choose a sample volume of Regarding sample volumes for prepacked columns, please refer to Sample preparation for analysis of proteins, peptides and carbohydrates selection guide.In group separation (desalting) the sample volumes can be up to 30% of the column volume.Ni Sepharose and IMAC Sepharose selection guide ![]() If the results are poor, refer to the symptom Poor packing evaluation in the troubleshooting section.Īffinity chromatography columns and media selection guide Please be aware of that for small columns, less than 10 ml bed volume, the system dead volume has an impact on the column evaluation values. Poorly packed bed (not sufficiently compressed during packing).Įvaluate the packing using recommended methods. Pre-filter or centrifuge sample to avoid residues building up. Disassemble the column and replace the support according to instructions. Increased resistance to flow due to blocked bed support compressing the packed bed.Ĭlean or change the bed support. Disassemble the column according to instructions.īuffer conditions deviate with regard to temperature, conductivity, viscosity, content of organic solvent (reduces surface tension) or other factor.Ĭheck the buffers and choose more suitable conditions. Can I reuse the column?īed support damaged or incorrectly assembled allowing chromatography medium particles to leave the column.Ĭheck the bed support and replace if necessary. My column has a gap between the packed bed and adaptor. Open any that is not fully open.įind more causes and remedies in the troubleshooting section. ![]() Sample and collection vessels at different levels.Īdjust the vessels to approximately the same level.Ĭheck all valves. The buffer normally become opalescent due to microbial growth.Ĭheck buffers, especially those with phosphate, for microbial growth. (Lower temperature gives higher viscosity.) Let low-temperature buffer reach operating temperature before starting the run. Repair/replace if necessary.Ĭlean the column according to instructions.Ĭhoose the more rigorous cleaning protocol when available.Ĭheck that the flow path is not restricted.Ĭheck the viscosity of all buffers. ReadyToProcess Capto adhere columns, 1 L and larger columns for clinical manufacturing that minimize the need for cleaning and cleaning validation.The backpressure increases during operationĪuxiliary equipment such as manometers and pumps not working properly.Ĭheck the function of all auxiliary equipment. Process Characterization Kit Capto adhere, designed for studying the potential impact that resin ligand density might have on the process outcome. HiTrap Capto adhere columns, for easy use with a syringe, peristaltic pump, or chromatography system. Lab scale: prepacked for automated purification and analysis Lab scale: prepacked for manual purification
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